Plasmid
pUC19 ori

Part:BBa_I50020:Design

Designed by: Reshma Shetty   Group:   (2006-01-09)

Minimal pUC19-derived high copy replication origin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 19
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was designed to be a near minimal pUC19-derived high copy replication origin for use in the designed BioBrick base vector BBa_I51000. Several mutations were introduced to eliminate useful restriction sites.

Source

pSB1A3 served as the basis of the design of BBa_I50022.

References

<biblio>

  1. Vieira-Gene-1982 pmid=6295879
  2. Norrander-Gene-1983 pmid=6323249
  3. Yanisch-Perron-Gene-1985 pmid=2985470
  4. Cabello-Nature-1976 pmid=765836
  5. Ioannou-Nat-Genet-1994 pmid=8136839

</biblio>